Description

Series GSE13394

Status Public on Nov 14 2008

Title A translational profiling approach for the molecular characterization of CNS cell types

http://www.ncbi.nlm.nih.gov/projects/geo/query/acc.cgi?acc=GSE13394

Organism(s) Mus musculus

Type Expression profiling by array
Summary The cellular heterogeneity of the brain confounds efforts to elucidate the biological properties of distinct neuronal populations.
Using Bacterial Artificial Chromosome (BAC) transgenic mice which express EGFP-tagged ribosomal protein L10a in defined cell populations, we have developed a methodology to affinity purify polysomal mRNAs from genetically defined cell populations in the brain.
The utility of this approach is illustrated by the comparative analysis of four types of neurons, revealing hundreds of genes that distinguish these four cell populations.
We find that even two morphologically indistinguishable, intermixed subclasses of medium spiny neuron display vastly different translational profiles and present examples of the physiological significance of such differences.
This genetically targeted Translating Ribosome Affinity Purification (TRAP) methodology is a generalizable method useful for the identification of molecular changes in any genetically defined cell type in response to genetic alterations, disease, or pharmacological perturbations.

This SuperSeries is composed of the following subset Series:
GSE13384: Comparative analysis of Drd1+ Medium Spiny Neurons and Drd2+ Medium Spiny Neurons
GSE13385: Comparative analysis of Drd1+ Medium Spiny Neurons, Drd2+ Medium Spiny Neurons and whole brain
GSE13386: Comparative analysis of Drd1+ Medium Spiny Neurons, Drd2+ Medium Spiny Neurons, cocaine treatment
GSE13387: Comparative analysis of Drd1+ Medium Spiny Neurons, Drd2+ Medium Spiny Neurons, Motor Neurons, and Purkinje Neurons

Overall design Refer to individual Series

Web Link

Contributor(s) Heiman Myriam, Schaefer Anne, Gong Shiaoching, Peterson Jayms, Day Michelle, Ramsey E Keri, Suárez-Fariñas Mayte, Schwarz Cordelia, Stephan A Dietrich, Surmeier J D, Greengard Paul, Heintz Nathaniel

Citation(s)

Submission date Oct 29 2008

Contact name Heintz Nathaniel

E-mail(s) heintz@rockefeller.edu

Phone

Fax

URL

Organization name HHMI/Rockefeller University

Department

Lab

Street address 1230 York Ave, Box 260

City New York

State/province NY

ZIP/Postal code 10021

Country USA

Platforms (1)
GPL1261 Affymetrix GeneChip Mouse Genome 430 2.0 Array

Samples(63)