1 to 10 of 12 literature results

• Wza the translocon for E. coli capsular polysaccharides defines a new class of membrane protein.

  Many types of bacteria produce extracellular polysaccharides (EPSs). Some are secreted polymers and show only limited association with the cell surfac…

  Authors: Changjiang Dong, Konstantinos Beis, Jutta Nesper, Anne L Brunkan-Lamontagne, Bradley R Clarke, Chris Whitfield, James H Naismith

  Source: Nature. 2006 Nov 9

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• Overcoming the challenges of membrane protein crystallography.

  Membrane protein structural biology is still a largely unconquered area, given that approximately 25% of all proteins are membrane proteins and yet le…

  Authors: Elisabeth P Carpenter, Konstantinos Beis, Alexander D Cameron, So Iwata

  Source: Current opinion in structural biology. 2008 Oct

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• The structure of the efflux pump AcrB in complex with bile acid.

  Gastrointestinal bacteria, like Escherichia coli, must remove bile acid to survive in the gut. Bile acid removal in E. coli is thought to be mediated …

  Authors: David Drew, Mirjam M Klepsch, Simon Newstead, Ralf Flaig, Jan-Willem De Gier, So Iwata, Konstantinos Beis

  Source: Molecular membrane biology. 2008 Dec

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• The structure of NADH in the enzyme dTDP-d-glucose dehydratase (RmlB).

  The structure of Streptococcus suis serotype type 2 dTDP-d-glucose 4,6-dehydratase (RmlB) has been determined to 1.5 A resolution with its nicotinamid…

  Authors: Konstantinos Beis, Simon T M Allard, Adrian D Hegeman, Garib Murshudov, Douglas Philp, James H Naismith

  Source: Journal of the American Chemical Society. 2003 Oct 1

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• Crystal structures of Mycobacteria tuberculosis and Klebsiella pneumoniae UDP-galactopyranose mutase in the oxidised state and Klebsiella pneumoniae UDP-galactopyranose mutase in the (active) reduced state.

  Uridine diphosphogalactofuranose (UDP-Galf) is the precursor of the d-galactofuranose sugar found in bacterial and parasitic cell walls, including tho…

  Authors: Konstantinos Beis, Velupillai Srikannathasan, Huanting Liu, Stephen W B Fullerton, Vicki A Bamford, David A R Sanders, Chris Whitfield, Mike R McNeil, James H Naismith

  Source: Journal of molecular biology. 2005 May 13

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• Two-step purification of outer membrane proteins.

  Here, we describe a simple and efficient method for the purification of Escherichia coli outer membrane proteins. We have tested this protocol for the…

  Authors: Konstantinos Beis, Chris Whitfield, Ian Booth, James H Naismith

  Source: International journal of biological macromolecules. 2006 Aug 15

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• The 3D structure of a periplasm-spanning platform required for assembly of group 1 capsular polysaccharides in Escherichia coli.

  Capsular polysaccharides (CPSs) are essential virulence determinants of many pathogenic bacteria. Escherichia coli group 1 CPSs provide paradigms for …

  Authors: Richard F Collins, Konstantinos Beis, Changjiang Dong, Catherine H Botting, Catherine McDonnell, Robert C Ford, Bradley R Clarke, Chris Whitfield, James H Naismith

  Source: Proceedings of the National Academy of Sciences of the United States of America. 2007 Feb 13

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• Periplasmic protein-protein contacts in the inner membrane protein Wzc form a tetrameric complex required for the assembly of Escherichia coli group 1 capsules.

  The K antigenic capsular polysaccharide forms a structural layer, the capsule, on the surfaces of Escherichia coli cells. The capsule provides an impo…

  Authors: Richard F Collins, Konstantinos Beis, Bradley R Clarke, Robert C Ford, Martyn Hulley, James H Naismith, Chris Whitfield

  Source: The Journal of biological chemistry. 2006 Jan 27

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• Crystallization and preliminary X-ray diffraction analysis of Wza outer-membrane lipoprotein from Escherichia coli serotype O9a:K30.

  A novel integral membrane lipoprotein, Wza, from Escherichia coli serotype O9a:K30 has been purified and crystallized. Wza is required for the surface…

  Authors: Konstantinos Beis, Jutta Nesper, Chris Whitfield, James H Naismith

  Source: Acta crystallographica. Section D, Biological crystallography. 2004 Mar

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• Three-dimensional structure of Wza, the protein required for translocation of group 1 capsular polysaccharide across the outer membrane of Escherichia coli.

  Wza is a highly conserved multimeric outer membrane protein complex required for the surface expression of the serotype K30 group 1 capsular polysacch…

  Authors: Konstantinos Beis, Richard F Collins, Robert C Ford, Alhaji B Kamis, Chris Whitfield, James H Naismith

  Source: The Journal of biological chemistry. 2004 Jul 2

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