1 to 8 of 8 literature results

• Use of a combination of isotopically coded cross-linkers and isotopically coded N-terminal modification reagents for selective identification of inter-peptide crosslinks.

  Cross-linking combined with mass spectrometry has great potential for determining three-dimensional structures of proteins and protein assemblies. One…

  Authors: Evgeniy V Petrotchenko, Jason J Serpa, Christoph H Borchers

  Source: Analytical chemistry. 2010 Feb 1

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• Combining fluorescence detection and mass spectrometric analysis for comprehensive and quantitative analysis of redox-sensitive cysteines in native membrane proteins.

  Monobromobimane (MBB) is a lipophilic reagent that selectively modifies free cysteine residues in proteins. Because of its lipophilic character, MBB i…

  Authors: Evgeniy V Petrotchenko, Dan Pasek, Phillip Elms, Nikolay V Dokholyan, Gerhard Meissner, Christoph H Borchers

  Source: Analytical chemistry. 2006 Dec 1

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• ICC-CLASS: isotopically-coded cleavable crosslinking analysis software suite.

  BACKGROUND: Successful application of crosslinking combined with mass spectrometry for studying proteins and protein complexes requires specifically-d…

  Authors: Evgeniy V Petrotchenko, Christoph H Borchers

  Source: BMC bioinformatics. 2010

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• Crystallographic analysis of a hydroxylated polychlorinated biphenyl (OH-PCB) bound to the catalytic estrogen binding site of human estrogen sulfotransferase.

  Certain hydroxylated polychlorinated biphenyls (OH-PCBs) inhibit the human estrogen sulfotransferase (hEST) at subnanomolar concentrations, suggesting…

  Authors: Sergei Shevtsov, Evgeniy V Petrotchenko, Lars C Pedersen, Masahiko Negishi

  Source: Environmental health perspectives. 2003 Jun

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• Inhibition of APCCdh1 activity by Cdh1/Acm1/Bmh1 ternary complex formation.

  The anaphase-promoting complex (APC) is an essential E3 ubiquitin ligase responsible for catalyzing proteolysis of key regulatory proteins in the cell…

  Authors: J Michael Dial, Evgeniy V Petrotchenko, Christoph H Borchers

  Source: The Journal of biological chemistry. 2007 Feb 23

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• BiPS, a photocleavable, isotopically coded, fluorescent cross-linker for structural proteomics.

  Cross-linking combined with mass spectrometry is an emerging approach for studying protein structure and protein-protein interactions. However, unambi…

  Authors: Evgeniy V Petrotchenko, Kunhong Xiao, Jennifer Cable, Yiwen Chen, Nikolay V Dokholyan, Christoph H Borchers

  Source: Molecular & cellular proteomics : MCP. 2009 Feb

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• Isotopically coded cleavable cross-linker for studying protein-protein interaction and protein complexes.

  An emerging approach for studying protein-protein interaction in complexes is the combination of chemical cross-linking and mass spectrometric analysi…

  Authors: Evgeniy V Petrotchenko, Vyacheslav K Olkhovik, Christoph H Borchers

  Source: Molecular & cellular proteomics : MCP. 2005 Aug

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• Combined top-down and bottom-up proteomics identifies a phosphorylation site in stem-loop-binding proteins that contributes to high-affinity RNA binding.

  The stem-loop-binding protein (SLBP) is involved in multiple aspects of histone mRNA metabolism. To characterize the modification status and sites of …

  Authors: Christoph H Borchers, Roopa Thapar, Evgeniy V Petrotchenko, Matthew P Torres, J Paul Speir, Michael Easterling, Zbigniew Dominski, William F Marzluff

  Source: Proceedings of the National Academy of Sciences of the United States of America. 2006 Feb 28

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