1 to 10 of 27 literature results

• Accurate sampling of high-frequency motions in proteins by steady-state (15)N-{(1)H} nuclear Overhauser effect measurements in the presence of cross-correlated relaxation.

  The steady-state {(1)H}-(15)N NOE experiment is used in most common NMR analyses of backbone dynamics to accurately ascertain the effects of the fast …

  Authors: Fabien Ferrage, David Cowburn, Ranajeet Ghose

  Source: Journal of the American Chemical Society. 2009 May 6

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• Protein backbone dynamics through 13C'-13Calpha cross-relaxation in NMR spectroscopy.

  Internal dynamics of proteins are usually characterized by the analysis of (15)N relaxation rates that reflect the motions of NH(N) vectors. It was su…

  Authors: Fabien Ferrage, Philippe Pelupessy, David Cowburn, Geoffrey Bodenhausen

  Source: Journal of the American Chemical Society. 2006 Aug 30

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• A full-length group 1 bacterial sigma factor adopts a compact structure incompatible with DNA binding.

  The sigma factors are the key regulators of bacterial transcription initiation. Through direct read-out of promoter DNA sequence, they recruit the cor…

  Authors: Edmund C Schwartz, Alexander Shekhtman, Kaushik Dutta, Matthew R Pratt, David Cowburn, Seth Darst, Tom W Muir

  Source: Chemistry & biology. 2008 Oct 20

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• Optimized bacterial expression and purification of the c-Src catalytic domain for solution NMR studies.

  Progression of a host of human cancers is associated with elevated levels of expression and catalytic activity of the Src family of tyrosine kinases (…

  Authors: Andrea Piserchio, Ranajeet Ghose, David Cowburn

  Source: Journal of biomolecular NMR. 2009 Jun

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• Structural determination of biomolecular interfaces by nuclear magnetic resonance of proteins with reduced proton density.

  Protein interactions are important for understanding many molecular mechanisms underlying cellular processes. So far, interfaces between interacting p…

  Authors: Fabien Ferrage, Kaushik Dutta, Alexander Shekhtman, David Cowburn

  Source: Journal of biomolecular NMR. 2010 May

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• N-terminal cysteinyl proteins can be prepared using thrombin cleavage.

  Expressed protein ligation--which allows native proteins to be selectively linked together by a normal peptide bond in an aqueous environment--has eme…

  Authors: Dongsheng Liu, Rong Xu, Kaushik Dutta, David Cowburn

  Source: FEBS letters. 2008 Apr 2

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• Segmental isotopic labeling of proteins for nuclear magnetic resonance.

  Nuclear magnetic resonance (NMR) spectroscopy has emerged as one of the principle techniques of structural biology. It is not only a powerful method f…

  Authors: Dongsheng Liu, Rong Xu, David Cowburn

  Source: Methods in enzymology. 2009

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• A cell-penetrating helical peptide as a potential HIV-1 inhibitor.

  The capsid domain of the human immunodeficiency virus type 1 (HIV-1) Gag polyprotein is a critical determinant of virus assembly, and is therefore a p…

  Authors: Hongtao Zhang, Qian Zhao, Shibani Bhattacharya, Abdul A Waheed, Xiaohe Tong, Anita Hong, Susanne Heck, Francesca Curreli, Michael Goger, David Cowburn,…

  Source: Journal of molecular biology. 2008 May 2

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• Nuclear spin relaxation in isotropic and anisotropic media.

  Authors: Matthew P Nicholas, Ertan Eryilmaz, Fabien Ferrage, David Cowburn, Ranajeet Ghose

  Source: Progress in nuclear magnetic resonance spectroscopy. 2010 Aug

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• Solution structure and folding characteristics of the C-terminal SH3 domain of c-Crk-II.

  Crk-II is a signaling adaptor protein that is involved in many cellular processes including apoptosis, proliferation, and differentiation. It has a mo…

  Authors: Vasant Muralidharan, Kaushik Dutta, Jaehyun Cho, Miquel Vila-Perello, Daniel P Raleigh, David Cowburn, Tom W Muir

  Source: Biochemistry. 2006 Jul 25

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