10 literature results

• Crystal structure of the pyocyanin biosynthetic protein PhzS.

  The human pathogen Pseudomonas aeruginosa produces pyocyanin, a blue-pigmented phenazine derivative, which is known to play a role in virulence. Pyocy…

  Authors: Bryan T Greenhagen, Katherine Shi, Howard Robinson, Swarna Gamage, Asim K Bera, Jane E Ladner, James F Parsons

  Source: Biochemistry. 2008 May 13

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• Structural and functional analysis of the pyocyanin biosynthetic protein PhzM from Pseudomonas aeruginosa.

  Pyocyanin is a biologically active phenazine produced by the human pathogen Pseudomonas aeruginosa. It is thought to endow P. aeruginosa with a compet…

  Authors: James F Parsons, Bryan T Greenhagen, Katherine Shi, Kelly Calabrese, Howard Robinson, Jane E Ladner

  Source: Biochemistry. 2007 Feb 20

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• Surrogate splicing for functional analysis of sesquiterpene synthase genes.

  A method for the recovery of full-length cDNAs from predicted terpene synthase genes containing introns is described. The approach utilizes Agrobacter…

  Authors: Shuiqin Wu, Mark A Schoenbeck, Bryan T Greenhagen, Shunji Takahashi, Sungbeom Lee, Robert M Coates, Joseph Chappell

  Source: Plant physiology. 2005 Jul

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• Metabolic engineering of sesquiterpene metabolism in yeast.

  Terpenes are structurally diverse compounds that are of interest because of their biological activities and industrial value. These compounds consist …

  Authors: Shunji Takahashi, Yunsoo Yeo, Bryan T Greenhagen, Tom McMullin, Linsheng Song, Julie Maurina-Brunker, Reinhardt Rosson, Joseph P Noel, Joe Chappell

  Source: Biotechnology and bioengineering. 2007 May 1

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• Probing sesquiterpene hydroxylase activities in a coupled assay with terpene synthases.

  5-epi-Aristolochene dihydroxylase (EAH) catalyzes unique stereo- and regiospecific hydroxylations of a bicyclic sesquiterpene hydrocarbon to generate …

  Authors: Bryan T Greenhagen, Paul Griggs, Shunji Takahashi, Lyle Ralston, Joe Chappell

  Source: Archives of biochemistry and biophysics. 2003 Jan 15

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• Identifying and manipulating structural determinates linking catalytic specificities in terpene synthases.

  Terpene synthases are a mechanistically intriguing family of enzymes that catalyze complex, multistep reactions that are capable of generating hundred…

  Authors: Bryan T Greenhagen, Paul E O'Maille, Joseph P Noel, Joe Chappell

  Source: Proceedings of the National Academy of Sciences of the United States of America. 2006 Jun 27

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• Quantitative exploration of the catalytic landscape separating divergent plant sesquiterpene synthases.

  Throughout molecular evolution, organisms create assorted chemicals in response to varying ecological niches. Catalytic landscapes underlie metabolic …

  Authors: Paul E O'Maille, Arthur Malone, Nikki Dellas, B Andes Hess, Lidia Smentek, Iseult Sheehan, Bryan T Greenhagen, Joe Chappell, Gerard Manning, Joseph P Noel

  Source: Nature chemical biology. 2008 Oct

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• Functional characterization of premnaspirodiene oxygenase, a cytochrome P450 catalyzing regio- and stereo-specific hydroxylations of diverse sesquiterpene substrates.

  Solavetivone, a potent antifungal phytoalexin, is derived from a vetispirane-type sesquiterpene, premnaspirodiene, by a putative regio- and stereo-spe…

  Authors: Shunji Takahashi, Yun-Soo Yeo, Yuxin Zhao, Paul E O'Maille, Bryan T Greenhagen, Joseph P Noel, Robert M Coates, Joe Chappell

  Source: The Journal of biological chemistry. 2007 Oct 26

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• Kinetic and molecular analysis of 5-epiaristolochene 1,3-dihydroxylase, a cytochrome P450 enzyme catalyzing successive hydroxylations of sesquiterpenes.

  The final step of capsidiol biosynthesis is catalyzed by 5-epiaristolochene dihydroxylase (EAH), a cytochrome P450 enzyme that catalyzes the regio- an…

  Authors: Shunji Takahashi, Yuxin Zhao, Paul E O'Maille, Bryan T Greenhagen, Joseph P Noel, Robert M Coates, Joe Chappell

  Source: The Journal of biological chemistry. 2005 Feb 4

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• Gene library synthesis by structure-based combinatorial protein engineering.

  Authors: Paul E O'Maille, Ming-Daw Tsai, Bryan T Greenhagen, Joseph Chappell, Joseph P Noel

  Source: Methods in enzymology. 2004

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