1 to 10 of 34 literature results

• Characterization of nonenzymatic glycation on a monoclonal antibody.

  We present here an improved analytical method for the analysis of glycation events in proteins. Nonenzymatic glycation of an IgG2 monoclonal antibody …

  Authors: Lowell J Brady, Theresa Martinez, Alain Balland

  Source: Analytical chemistry. 2007 Dec 15

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• Hydrophobic interaction chromatography of soluble interleukin I receptor type II to reveal chemical degradations resulting in loss of potency.

  A hydrophobic interaction chromatography method was developed to analyze recombinant soluble Interleukin 1 receptor type II (sIL-1R type II) drug subs…

  Authors: Yuling Zhang, Theresa Martinez, Brian Woodruff, Andy Goetze, Robert Bailey, Dean Pettit, Alain Balland

  Source: Analytical chemistry. 2008 Sep 15

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• Fluorescent derivatization method of proteins for characterization by capillary electrophoresis-sodium dodecyl sulfate with laser-induced fluorescence detection.

  A fast and improved sample preparation scheme was developed for protein analysis using capillary electrophoresis-sodium dodecyl sulfate (CE-SDS) with …

  Authors: David A Michels, Lowell J Brady, Amy Guo, Alain Balland

  Source: Analytical chemistry. 2007 Aug 1

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• Separation and characterization of an IgG2 antibody containing a cyclic imide in CDR1 of light chain by hydrophobic interaction chromatography and mass spectrometry.

  Hydrophobic interaction chromatography (HIC) was used to separate populations of recombinant IgG2 antibody that were created as a result of prolonged …

  Authors: John Valliere-Douglass, Laura Jones, Diana Shpektor, Paul Kodama, Alison Wallace, Alain Balland, Robert Bailey, Yuling Zhang

  Source: Analytical chemistry. 2008 May 1

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• Molecular mass analysis of antibodies by on-line SEC-MS.

  Mass analysis of recombinant protein therapeutics is an important assay for product characterization. Intact mass analysis is used to provide confirma…

  Authors: Lowell J Brady, John Valliere-Douglass, Theresa Martinez, Alain Balland

  Source: Journal of the American Society for Mass Spectrometry. 2008 Apr

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• Interchain disulfide bonding in human IgG2 antibodies probed by site-directed mutagenesis.

  Human IgG2 exists as a mixture of disulfide-linked structural isoforms that can show different activities. To probe the contribution of specific cyste…

  Authors: Martin J Allen, Amy Guo, Theresa Martinez, Mei Han, Gregory C Flynn, Jette Wypych, Yaoqing Diana Liu, Wenyan D Shen, Thomas M Dillon, Christopher Vezina,…

  Source: Biochemistry. 2009 May 5

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• O-fucosylation of an antibody light chain: characterization of a modification occurring on an IgG1 molecule.

  We describe the characterization of an O-fucosyl modification to a serine residue on the light chain of a recombinant, human IgG1 molecule expressed i…

  Authors: John F Valliere-Douglass, Lowell J Brady, Chris Farnsworth, Danielle Pace, Alain Balland, Alison Wallace, Wesley Wang, Michael J Treuheit, Boxu Yan

  Source: Glycobiology. 2009 Feb

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• Electrophoretic evidence for the presence of structural isoforms specific for the IgG2 isotype.

  Recombinant monoclonal antibodies of therapeutic interest were analyzed by a nonreduced CE-SDS (nrCE-SDS) method developed for the evaluation of size-…

  Authors: Amy Guo, Mei Han, Theresa Martinez, Randal R Ketchem, Shawn Novick, Claudia Jochheim, Alain Balland

  Source: Electrophoresis. 2008 Jun

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• Disulfide connectivity of human immunoglobulin G2 structural isoforms.

  In this communication we present the detailed disulfide structure of IgG2 molecules. The consensus structural model of human IgGs represents the hinge…

  Authors: Theresa Martinez, Amy Guo, Martin J Allen, Mei Han, Danielle Pace, Jay Jones, Ron Gillespie, Randal R Ketchem, Yuling Zhang, Alain Balland

  Source: Biochemistry. 2008 Jul 15

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• Succinimide formation at Asn 55 in the complementarity determining region of a recombinant monoclonal antibody IgG1 heavy chain.

  We investigated the formation and stability of succinimide, an intermediate of deamidation events, in recombinant monoclonal antibodies (mAbs). During…

  Authors: Boxu Yan, Sean Steen, David Hambly, John Valliere-Douglass, Tim Vanden Bos, Scott Smallwood, Zac Yates, Thomas Arroll, Yihong Han, Himanshu Gadgil,…

  Source: Journal of pharmaceutical sciences. 2009 Oct

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