Clear Search sequence regions


  • cell (1)
  • chromatin (4)
  • gene (3)
  • heterochromatin (3)
  • mice (1)
  • movement (1)
  • myogenesis (1)
  • nuclear lamina (5)
  • regions (2)
  • signals (1)
  • stem cell (5)
  • Sizes of these terms reflect their relevance to your search.

    Progenitor cells differentiate into specialized cell types through coordinated expression of lineage-specific genes and modification of complex chromatin configurations. We demonstrate that a histone deacetylase (Hdac3) organizes heterochromatin at the nuclear lamina during cardiac progenitor lineage restriction. Specification of cardiomyocytes is associated with reorganization of peripheral heterochromatin, and independent of deacetylase activity, Hdac3 tethers peripheral heterochromatin containing lineage-relevant genes to the nuclear lamina. Deletion of Hdac3 in cardiac progenitor cells releases genomic regions from the nuclear periphery, leading to precocious cardiac gene expression and differentiation into cardiomyocytes; in contrast, restricting Hdac3 to the nuclear periphery rescues myogenesis in progenitors otherwise lacking Hdac3. Our results suggest that availability of genomic regions for activation by lineage-specific factors is regulated in part through dynamic chromatin-nuclear lamina interactions and that competence of a progenitor cell to respond to differentiation signals may depend upon coordinated movement of responding gene loci away from the nuclear periphery. Copyright © 2017 Elsevier Inc. All rights reserved.

    Citation

    Andrey Poleshko, Parisha P Shah, Mudit Gupta, Apoorva Babu, Michael P Morley, Lauren J Manderfield, Jamie L Ifkovits, Damelys Calderon, Haig Aghajanian, Javier E Sierra-Pagán, Zheng Sun, Qiaohong Wang, Li Li, Nicole C Dubois, Edward E Morrisey, Mitchell A Lazar, Cheryl L Smith, Jonathan A Epstein, Rajan Jain. Genome-Nuclear Lamina Interactions Regulate Cardiac Stem Cell Lineage Restriction. Cell. 2017 Oct 19;171(3):573-587.e14

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 29033129

    View Full Text