Clear Search sequence regions

  • biophys (1)
  • biosensor (1)
  • commun (1)
  • dna (4)
  • dna binds (1)
  • dna sequence (4)
  • facilitates (1)
  • nucleotides (1)
  • rna (3)
  • rna sequence (3)
  • Sizes of these terms reflect their relevance to your search.

    A unique specificity of the aptamer-ligand biorecognition and binding facilitates bioanalysis and biosensor development, contributing to discrimination of structurally related molecules, such as dopamine and other catecholamine neurotransmitters. The aptamer sequence capable of specific binding of dopamine is a 57 nucleotides long RNA sequence reported in 1997 (Biochemistry, 1997, 36, 9726). Later, it was suggested that the DNA homologue of the RNA aptamer retains the specificity of dopamine binding (Biochem. Biophys. Res. Commun., 2009, 388, 732). Here, we show that the DNA sequence obtained by the replacement of the RNA aptamer bases for their DNA analogues is not able of specific biorecognition of dopamine, in contrast to the original RNA aptamer sequence. This DNA sequence binds dopamine and structurally related catecholamine neurotransmitters non-specifically, as any DNA sequence, and, thus, is not an aptamer and cannot be used neither for in vivo nor in situ analysis of dopamine in the presence of structurally related neurotransmitters. Copyright © 2017 Elsevier Inc. All rights reserved.


    Isabel Álvarez-Martos, Elena E Ferapontova. A DNA sequence obtained by replacement of the dopamine RNA aptamer bases is not an aptamer. Biochemical and biophysical research communications. 2017 Aug 05;489(4):381-385

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 28576492

    View Full Text