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Photochromic ultrafast reactions of bacteriorhodopsin (H. salinarum) and bovine rhodopsin were conducted with a femtosecond two-pump probe pulse setup with the time resolution of 20-25fs. The dynamics of the forward and reverse photochemical reactions for both retinal-containing proteins was compared. It is demonstrated that when retinal-containing proteins are excited by femtosecond pulses, dynamics pattern of the vibrational coherent wave packets in the course of the reaction is different for bacteriorhodopsin and visual rhodopsin. As shown in these studies, the low-frequencies that form a wave packets experimentally observed in the dynamics of primary products formation as a result of retinal photoisomerization have different intensities and are clearer for bovine rhodopsin. Photo-reversible reactions for both retinal proteins were performed from the stage of the relatively stable photointermediates that appear within 3-5ps after the light pulse impact. It is demonstrated that the efficiency of the reverse phototransition K-form→bacteriorhodopsin is almost five-fold higher than that of the Batho-intermediate→visual rhodopsin phototransition. The results obtained indicate that in the course of evolution the intramolecular mechanism of the chromophore-protein interaction in visual rhodopsin becomes more perfect and specific. The decrease in the probability of the reverse chromophore photoisomerization (all-trans→11-cis retinal) in primary photo-induced rhodopsin products causes an increase in the efficiency of the photoreception process. Copyright © 2016 Elsevier B.V. All rights reserved.

Citation

Tatiana B Feldman, Olga A Smitienko, Ivan V Shelaev, Fedor E Gostev, Oksana V Nekrasova, Dmitriy A Dolgikh, Victor A Nadtochenko, Mikhail P Kirpichnikov, Mikhail A Ostrovsky. Femtosecond spectroscopic study of photochromic reactions of bacteriorhodopsin and visual rhodopsin. Journal of photochemistry and photobiology. B, Biology. 2016 Nov;164:296-305

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PMID: 27723489

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