Clear Search sequence regions


  • adult (1)
  • after treatment (1)
  • aids vaccines (2)
  • apoptosis (1)
  • cells (9)
  • copies (2)
  • cytolysis (2)
  • e mail (1)
  • humans (1)
  • phase (1)
  • plasma (4)
  • rna (3)
  • t lymphocytes (2)
  • viral load (1)
  • viremia (2)
  • virus (1)
  • Sizes of these terms reflect their relevance to your search.

    We report the results of a phase I/II, open-label, single-arm clinical trial to evaluate the safety and anti-human immunodeficiency virus type 1 (HIV-1) efficacy of an autologous dendritic cell (DC)-based HIV-1 vaccine loaded with autologous HIV-1-infected apoptotic cells. Antiretroviral therapy (ART)-naive individuals were enrolled, and viremia was suppressed by ART prior to delivery of 4 doses of DC-based vaccine. Participants underwent treatment interruption 6 weeks after the third vaccine dose. The plasma HIV-1 RNA level 12 weeks after treatment interruption was compared to the pre-ART (ie, baseline) level. The vaccine was safe and well tolerated but did not prevent viral rebound during treatment interruption. Vaccination resulted in a modest but significant decrease in plasma viremia from the baseline level (from 4.53 log10 copies/mL to 4.27 log10 copies/mL;P= .05). Four of 10 participants had a >0.70 log10 increase in the HIV-1 RNA load in plasma following vaccination, despite continuous ART. Single-molecule sequencing of HIV-1 RNA in plasma before and after vaccination revealed increases in G>A hypermutants in gag and pol after vaccination, which suggests cytolysis of infected cells. A therapeutic HIV-1 vaccine based on DCs loaded with apoptotic bodies was safe and induced T-cell activation and cytolysis, including HIV-1-infected cells, in a subset of study participants. NCT00510497. © The Author 2015. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail journals.permissions@oup.com.

    Citation

    Bernard J C Macatangay, Sharon A Riddler, Nicole D Wheeler, Jonathan Spindler, Mariam Lawani, Feiyu Hong, Mary J Buffo, Theresa L Whiteside, Mary F Kearney, John W Mellors, Charles R Rinaldo. Therapeutic Vaccination With Dendritic Cells Loaded With Autologous HIV Type 1-Infected Apoptotic Cells. The Journal of infectious diseases. 2016 May 1;213(9):1400-9

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 26647281

    View Full Text