Clear Search sequence regions


  • cell (1)
  • chromosomes d (3)
  • dna (1)
  • electron (4)
  • face (3)
  • human chromosomes (5)
  • humans (1)
  • male (1)
  • mitosis (1)
  • osmium (1)
  • platinum (3)
  • resin (1)
  • Sizes of these terms reflect their relevance to your search.

    The high-order structure of human chromosomes is an important biological question that is still under investigation. Studies have been done on imaging human mitotic chromosomes using mostly 2-D microscopy methods. To image micron-sized human chromosomes in 3-D, we developed a procedure for preparing samples for serial block-face scanning electron microscopy (SBFSEM). Polyamine chromosomes are first separated using a simple filtration method and then stained with heavy metal. We show that the DNA-specific platinum blue provides higher contrast than osmium tetroxide. A two-step procedure for embedding chromosomes in resin is then used to concentrate the chromosome samples. After stacking the SBFSEM images, a familiar X-shaped chromosome was observed in 3-D.

    Citation

    Mohammed Yusuf, Bo Chen, Teruo Hashimoto, Ana Katrina Estandarte, George Thompson, Ian Robinson. Staining and embedding of human chromosomes for 3-d serial block-face scanning electron microscopy. BioTechniques. 2014 Dec;57(6):302-7

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 25495730

    View Full Text