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To clone the Noxa gene and to observe the apoptosis of A549 cells transfected with the recombinant plasmid of pcDNA-Noxa. The Noxa gene was obtained by PCR, and was cloned into pcDNA3. 1(-). A549 cells were transfected with the recombinant plasmid of pcDNA-Noxa. Western blot analysis was performed to determine the overexpression of Noxa. A549 cells were stained with Hoechst 33258 to observe the apoptosis. The recombinant plasmid of pcDNA-Noxa was successfully constructed evidenced by endonuclease digestion and sequence analysis. The overexpression of Noxa was identified using Western blot analysis. The recombinant plasmid of pcDNA-Noxa induced apoptosis of A549 cells. Nora has exhibited potential pro-apoptotic activity against A549 cells. This study is a foundation for further research into pro-apoptotic activity of Noxa gene.

Citation

Deng-bang Chen, Kang Cao, Jing Yang, Qu Pan, Tian Chen, Jin-Chuan Li, Xin Wang, Jian-Hua Yin, Yi Zou, Chang-You Wei. Apoptosis of A549 cells induced by cloned Noxa gene]. Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition. 2013 Sep;44(5):713-6, 726

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PMID: 24325096

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