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To provide a tool for research on regulating adipocyte differentiation, tetracycline inducible (Tet on) lentiviral expression vectors under the control of an adipose-specific promoter were constructed. The lowest basal expression in the absence of doxycycline and most efficient dose-dependent, doxycycline-induced transient overexpression was observed using vectors constructed with a combination of Tetracycline Responsive Element (TRE) and reverse tetracycline-controlled TransActivator advanced (rtTAadv), transfected in white (3T3-L1) and brown (HIB-1B) preadipocytes cell lines. The results demonstrate that doxycycline adipogenic inducible expression can be achieved using a pLenti TRE / rtTA adv under the control of the truncated aP2 promoter in HIB-1B preadipocytes. Copyright © 2012 Elsevier Inc. All rights reserved.

Citation

Q Liu, P J Hill, A Karamitri, K J P Ryan, H Y Chen, M A Lomax. Construction of a doxycycline inducible adipogenic lentiviral expression system. Plasmid. 2013 Jan;69(1):96-103

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PMID: 23099229

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