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ARHGAP21 is a 217 kDa RhoGAP protein shown to modulate cell migration through the control of Cdc42 and FAK activities. In the present work a 250 kDa-ARHGAP21 was identified by mass spectrometry. This modified form is differentially expressed among cell lines and human primary cells. Co-immunoprecipitations and in vitro SUMOylation confirmed ARHGAP21 specific modification by SUMO2/3 and mapped the SUMOylation site to ARHGAP21 lysine K1443. Immunofluorescence staining revealed that ARHGAP21 co-localizes with SUMO2/3 in the cytoplasm and membrane compartments. Interestingly, our results suggest that ARHGAP21 SUMOylation may be related to cell proliferation. Therefore, SUMOylation of ARHGAP21 may represent a way of guiding its function. Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.


Carolina L Bigarella, Karla P Vieira Ferro, Karin S A Barcellos, Daniel Martins-de-Souza, Fabiola Traina, José C Novello, Sara T Olalla Saad, Leticia Fröhlich Archangelo. Post-translational modification of the RhoGTPase activating protein 21, ARHGAP21, by SUMO2/3. FEBS letters. 2012 Sep 21;586(19):3522-8

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PMID: 22922005

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