Clear Search sequence regions


A new actinomycete strain designated as Streptomyces sp. CTF15 was isolated from a saline soil using casein-KNO(3) agar medium. The strain Streptomyces sp. CTF15 exhibited promising antimicrobial activity against Staphylococcus aureus, Bacillus subtilis, Streptomyces viridochromogens Tu57 and high cytotoxicity (91.2% mortality) against Artimia salina in biological screening. The cultivation of this strain in a 50 L lab fermenter and subsequent isolation and purification by a series of chromatographic techniques and structure elucidation by MS and NMR analysis of the active metabolites revealed that it is a highly stable producer of resistomycin (1), tetracenomycin D (2) and actinomycin D (3), even under non-optimised culture conditions. The morphological, microscopic, biochemical and physiological characterisation suggested that the strain CTF15 belongs to the genus Streptomyces. A partial 16S rRNA gene sequence (1429 bp) from the strain CTF15 was determined and found to have high identity (99%) with Streptomyces griseoincarnatus. As such, this is the first report of a strain of S. griseoincarnatus capable of producing these three bioactive compounds simultaneously.

Citation

Imran Sajid, Khaled A Shaaban, Shahida Hasnain. Antitumour compounds from a saline soil isolate, Streptomyces griseoincarnatus CTF15. Natural product research. 2011 Mar;25(5):549-59

Expand section icon Mesh Tags

Expand section icon Substances


PMID: 21391117

View Full Text