Clear Search sequence regions

Small RNAs bound to Argonaute proteins recognize partially or fully complementary nucleic acid targets in diverse gene-silencing processes. A subgroup of the Argonaute proteins--known as the 'Piwi family'--is required for germ- and stem-cell development in invertebrates, and two Piwi members--MILI and MIWI--are essential for spermatogenesis in mouse. Here we describe a new class of small RNAs that bind to MILI in mouse male germ cells, where they accumulate at the onset of meiosis. The sequences of the over 1,000 identified unique molecules share a strong preference for a 5' uridine, but otherwise cannot be readily classified into sequence families. Genomic mapping of these small RNAs reveals a limited number of clusters, suggesting that these RNAs are processed from long primary transcripts. The small RNAs are 26-31 nucleotides (nt) in length--clearly distinct from the 21-23 nt of microRNAs (miRNAs) or short interfering RNAs (siRNAs)--and we refer to them as 'Piwi-interacting RNAs' or piRNAs. Orthologous human chromosomal regions also give rise to small RNAs with the characteristics of piRNAs, but the cloned sequences are distinct. The identification of this new class of small RNAs provides an important starting point to determine the molecular function of Piwi proteins in mammalian spermatogenesis.


Alexei Aravin, Dimos Gaidatzis, S├ębastien Pfeffer, Mariana Lagos-Quintana, Pablo Landgraf, Nicola Iovino, Patricia Morris, Michael J Brownstein, Satomi Kuramochi-Miyagawa, Toru Nakano, Minchen Chien, James J Russo, Jingyue Ju, Robert Sheridan, Chris Sander, Mihaela Zavolan, Thomas Tuschl. A novel class of small RNAs bind to MILI protein in mouse testes. Nature. 2006 Jul 13;442(7099):203-7

Expand section icon Mesh Tags

Expand section icon Substances

PMID: 16751777

View Full Text